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Buffers & Accessories



Buffers 
NuSep buffers are designed to make your research easier and more consistent. All buffers are made from high purity reagents and are subject to strict quality controls. With our sample and running buffers you will get optimum results every time you use them.

NuSep's range of buffers for use include:
•  Tris-Glycine SDS Running Buffer 5L Sachet (Cat # BG-143) 
•  
Protein Load Buffer 1mL X 10 (2x) (Cat # BG-147)
•  Tris-Glycine Kit (containing BG-143 and BG-147) (Cat # BG-146) 
•  Tris-HEPES SDS Running Buffer 5L Sachet (Cat # BG-163) 
•  SingleShot Tris-Glycine Buffer (TG Buffer) 20 Pouches/Box (Cat # DBM-TRG-01)
•  SingleShot Tris-Glycine-SDS Buffer (TGS Buffer) 20 Pouches/Box (Cat # DBM-TGS-01)
•  SingleShot Tris-Acetate-EDTA Buffer (TAE Buffer) 20 Pouches/Box (Cat # DBM-TAE-01)
•  SingleShot Tris-Borate-EDTA Buffer (TBE Buffer) 20 Pouches/Box (Cat # DBM-TBE-01)
•  SingleShot Phosphate Buffered Saline (PBS Buffer) 20 Pouches/Box (Cat # DBM-PBS-01)
•  SingleShot LB Agar 20 Pouches/Box (Cat # DBM-LBA-01)
•  SingleShot LB Media 20 Pouches/Box (Cat # DBM-LBM-01)


 
Protein Molecular Weight Markers
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nUView Wide Range Protein Markers (Cat # GM-900) are designed for use with nUView PAGE gels. Protein markers cover a wide range of molecular weights from 200kDa to 6.5kDa.  NuSep protein markers are lyophilized with sample buffer so they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis. NuSep protein markers are compatible with common staining procedures and UV imaging.
 
  nUView Protein Marker - Wide Range, mol wt 6,500-200,000 Da
   Usage: Sufficient for 20-30 mini gels (10X10cm)
   Storage temp: 2-8C 
 
   Description
   Protein (mol. wt.)
   Aprotinin, bovine lung (6,500)
   alpha-lactalbumin, bovine milk (14,200)
   Trypsin inhibitor, soybean (20,000)
   Trypsinogen, bovine pancrease (24,000)
   Carbonic anhydrase, bovine dehydrogenase, rabbit muscle (29,000)
   Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
   Ovalbumin, chicken egg (45,0000)
   Glutamic dehydrogenase, bovine liver (55,000)
   Albumin, bovine serum (66,000)
   Phosphorylase B, rabbit muscle (97,000)
   beta-Galactosidase, E. coli (116,000)
   Myosin, porcine heart (200,000)
 
 
 
 Stains 

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NuSep Coomassie Stain Fast is a ready-to-use colloidal G-250 blue stain. It is a premixed nonhazardous solution specially formulated for sensitive detection of proteins and safe, nonhazardous disposal. It is the most sensitive Colloidal Coomassie stain on the market, with the ability to detect down to 1ng denatured BSA and contains no methanol, acetic acid or phosphoric acid.

NuSep Coomassie Stain Eco-Friendly is a ready-to-use colloidal G-250 blue stain. It is a premixed nonhazardous solution specially formulated for sensitive detection of proteins and safe, nonhazardous disposal. It is the most sensitive Colloidal Coomassie stain on the market, with the ability to detect less than 5ng denatured BSA It contains no methanol, acetic, phosphoric acid or other hazardous components.

NuSep's range of stains and accessories include:

•  
Coomassie Electrophoresis Stain Fast 1 Liter (Cat # SG-013)
•  Coomassie Electrophoresis Stain Eco-Friendly 1 Liter (Cat # SG-023)


 Accessories

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Transfer Membranes and Accessories

Convenient nitrocellulose, PVDF membranes and precut blot paper for Western blotting.

NuSep's range of transfer membranes and accessories include:

•  
Blot Paper-Wet (3cm x 8.5cm) Pk100 (Cat # RB-100)
•  Blot Paper-Semi Dry (3cm x 8.5cm) Pk50 (Cat # RB-200)
•  Blot Paper-Wet (7cm x 8.5cm) Pk100 (Cat # RB-300)
•  Transfer Membrane NC 0.45um (3cm x 8.5cm) Pk10 (Cat # RB-500)
•  Transfer Membrane NC 0.22um (7cm x 8.4cm) Pk10 (Cat # RB-700)
•  Transfer Membrane NC 0.45um (7cm x 8.5cm) Pk10 (Cat # RB-700)
•  Transfer Membrane PVDF 0.45um (3cm x 8.5cm) Pk10 (Cat # RB-800)
•  Transfer Membrane PVDF 0.2um (7cm x 8.5cm) Pk10 (Cat # RB-900)
•  Transfer Membrane PVDF 0.45um (7cm x 8.5cm) Pk10 (Cat # RB-1000)